HPLC Chiral Separation of Paclobutrazol Enantiomers on Cyralis AM Column

HPLC Chiral Method for Analysis of Paclobutrazol Enantiomers on Cyralis AM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Paclobutrazol.

Paclobutrazol (PBZ) is an organic fungicide with the chemical formula C15H20ClN3O. It works through inhibiting the production of gibberellins, which are plant growth hormones. Through reducing reducing internodal growth, it causes stronger stems, greater root growth, and earlier fruitset. It can also reduce frost sensitivity in plants. You can find detailed UV spectra of Paclobutrazol and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Paclobutrazol enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Paclobutrazol can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.

Condition

ColumnCyralis AM, 4.6 x 100 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/DEA
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution2.52

Description

Class of CompoundsFungicide
Analyzing CompoundsPaclobutrazol

Application Column

Cyralis AM

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Paclobutrazol
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.