HPLC Chiral Separation of Omeprazole Enantiomers on Cyralis CL Column

HPLC Chiral Method for Analysis of Omeprazole Enantiomers on Cyralis CL Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Omeprazole.

Omeprazole is a proton pump inhibitor (PPI) with the chemical formula C17H19N3O3S. It is a medication that decreases the amount of acid produced in the stomach.  Omeprazole has a bactericidal effect on Helicobacter pylori.

This application describes a normal-phase chiral HPLC method for the separation of [compound] enantiomers using SIELC’s Cyralis CL chiral column. Cyralis CL is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Omeprazole can be retained and analyzed using the Cyralis CL stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of sodium perchlorate and acetonitrile. Detection is performed using UV.

Condition

ColumnCyralis CL, 4.6 x 100 mm, 5 µm, 100 A, reversed phase
Mobile PhaseMeCN/100 mM Ammonium Carbonate pH 9
Flow Rate1.0 mL/min
DetectionUV 280 nm
Resolution2.14

Description

Class of CompoundsProton Pump Inhibitor
Analyzing CompoundsOmeprazole

Application Column

Cyralis CL

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: reversed phase

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Application Analytes:
Omeprazole
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.