HPLC Chiral Separation of Mandelic Acid on Cyralis CM Column

HPLC Method for Analysis of Mandelic Acid on Cyralis CM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Mandelic Acid.

Mandelic Acid is a an aromatic alpha hydroxy acid with the chemical formula C8H8O3. It is used primarily as a precursor in organic synthesis of various drugs. While it is also a common component of chemical face peels, it is not recommended as treatment for acne. You can find detailed UV spectra of Mandelic Acid and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Mandelic Acid enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Mandelic Acid can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, IPA, and TFA. Detection is performed using UV.

Condition

ColumnCyralis CM, 4.6 x 250 mm, 5 µm, 100 A, dual ended
Mobile PhaseHexane/IPA/TFA – 70/30/0.5%
Flow Rate1.0 mL/min
DetectionUV 230 nm
Resolution3.01

Description

Class of CompoundsAcid
Analyzing CompoundsMandelic Acid

Application Column

Cyralis CM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Mandelic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.