HPLC Method for Spinosyn A, Spinosyn D on Lipak by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Spinosyn A, Spinosyn D
Spinosyn A and Spinosyn D are two parts of an insecticide called “Spinosad”. The insecticide works through both contact and ingestion by numerous insect species. Spinosyns and spinodoids target and bind to sites on nicotinic acetylcholine receptors, which disrupts neurotransmission and leads to the insects dying by hyperexcitation of the nervous system.
The sample was created by diluting a milliliter of commercial Spinosad solution in acetonitrile using a 10 mL volumetric flask and mixing at high speed until all unnecessary content separated from the needed solution.
Spinosyn A, Spinosyn D can be retained and analyzed using the Lipak stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Lipak, 3.2 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 40-70%, 10 min |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 245 |
| Limit of Detection* | 0.2 ppm |
| Class of Compounds | Insecticide |
| Analyzing Compounds | Spinosyn A, Spinosyn D |
Application Column
Lipak
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Spinosyn D
