| CAS Number | 131929-63-0 |
|---|---|
| Molecular Formula | C42H67NO10 |
| Molecular Weight | 745.995 |
| InChI Key | RDECBWLKMPEKPM-LEUBSNHMSA-N |
| LogP | 4.25 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Spinosyn D
April 29, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Spinosyn D check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was dissolved in acetonitrile. This analysis was done with the mobile phase made of acetonitrile, water, and sulfuric acid.
HPLC Method for Spinosyn A and D in Pesticide Formulation on Lipak Colum
April 29, 2026
HPLC Method for Spinosyn A, Spinosyn D on Lipak by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Spinosyn A, Spinosyn D
Spinosyn A and Spinosyn D are two parts of an insecticide called “Spinosad”. The insecticide works through both contact and ingestion by numerous insect species. Spinosyns and spinodoids target and bind to sites on nicotinic acetylcholine receptors, which disrupts neurotransmission and leads to the insects dying by hyperexcitation of the nervous system.
The sample was created by diluting a milliliter of commercial Spinosad solution in acetonitrile using a 10 mL volumetric flask and mixing at high speed until all unnecessary content separated from the needed solution.
Spinosyn A, Spinosyn D can be retained and analyzed using the Lipak stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Lipak, 3.2 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 40-70%, 10 min |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 245 |
| Limit of Detection* | 0.2 ppm |
| Class of Compounds | Insecticide |
| Analyzing Compounds | Spinosyn A, Spinosyn D |
Application Column
Lipak
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Spinosyn D
Separation of Spinosyn D on Newcrom R1 HPLC column
February 16, 2018
Spinosyn D can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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