HPLC Method for Analysis of Myelin Basic Protein (85-99) Peptide Antagonist on Chromni Column

HPLC Method for Myelin Basic Protein Peptide Antagonist on Chromni by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Myelin Basic Protein Peptide Antagonist

Myelin Basic Protein Peptide Antagonist is a synthetic peptide antagonist with the molecular formula C70H114N18O21 • XCF3COOH. When expressing an MBP (85-99), it inhibits the production of IL-2 in T cell hybridoma cells. MBP peptide antagonist also delays MBP (85-99)-induced experimental autoimmune encephalomyelitis and reduces symptom severity. You can find detailed UV spectra of Myelin Basic Protein Peptide Antagonist and information about its various lambda maxima by visiting the following link.

Myelin Basic Protein Peptide Antagonist is analyzed using a Chromni stationary phase column under gradient conditions with a mobile phase composed of acetonitrile (MeCN) and water. Detection is performed by UV absorbance detection.

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O- 80/20 – 60/40%, 10 min
BufferH2SO4 – 0.2%
Flow Rate1.0 mL/min
DetectionUV 200 nm, UV 280 nm
Limit of Detection*200 nm – 46 ppb, 280 nm – 733.9 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Peptide
Analyzing CompoundsMyelin Basic Protein Peptide Antagonist

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Myelin Basic Protein Peptide Antagonist
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.