| CAS Number | 298-12-4 |
|---|---|
| Molecular Formula | C2H2O3 |
| Molecular Weight | 74.036 |
| InChI Key | HHLFWLYXYJOTON-UHFFFAOYSA-N |
| LogP | -0.789 |
| Synonyms |
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Applications:
UV-Vis Spectrum of Glyoxylic Acid
December 24, 2025
If you are looking for optimized HPLC method to analyze Glyoxylic acid check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Dicarbon Carboxylic Acids on Newcrom BH Column
June 6, 2023
HPLC Method for Analysis of Oxalic Acid, Glycolic acid, Acetic Acid, Glyoxylic acid on Newcrom BH by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
- Glyoxylic Acid (CHO-COOH): This is the smallest α-keto acid. It is a yellowish solid that occurs naturally and is useful industrially. It participates in many biochemical reactions. Its reduced form, D-lactic acid, can be found in various microorganisms.
- Glycolic Acid (HOCH2COOH): This is the smallest α-hydroxy acid (AHA). It’s derived from sugar cane and is a colorless, odorless, and hygroscopic crystalline solid. It is used in various skin-care products because of its capability to penetrate skin.
- Acetic Acid (CH3COOH): It is a simple carboxylic acid. It’s an important chemical reagent and industrial chemical, primarily used in the production of cellulose acetate for photographic film and polyvinyl acetate for wood glue, as well as synthetic fibers and fabrics. It gives vinegar its sour taste and pungent smell.
- Oxalic Acid (C2H2O4): This is a colorless crystalline solid that forms a colorless solution in water. It occurs naturally in many foods, but too much can lead to kidney stones. It’s used in many different industrial processes, including rust removal, bleaching, and as a reducing agent in developing photographic film.
These four acids be separate and analyzed on a reverse-phase Newcrom BH, 4.6 x 150 mm, 3 µm, 100 A, dual ended column with a mobile phase consisting of water and perchloric acid as a buffer modifier. This analysis method can be UV detected at 200 nm.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Oxalic Acid, Glycolic acid, Acetic Acid, Glyoxylic acid
Condition
| Column | Newcrom BH, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | H2O -99.7% |
| Buffer | HClO4 – 0.3 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
Description
| Class of Compounds | Dicarbon Carboxylic Acids |
| Analyzing Compounds | Oxalic Acid, Glycolic acid, Acetic Acid, Glyoxylic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Glycolic acid
Glyoxylic acid
Oxalic Acid
Separation of Glyoxylic acid on Newcrom R1 HPLC column
February 16, 2018
Glyoxylic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options