Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze gamma-Cyclodextrin check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.

HPLC Method for beta-CYCLODEXTRIN, alpha-CYCLODEXTRIN, gamma-Cyclodextrin on Primesep S2 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of B-Cyclodextrin, G-Cyclodextrin and A-Cyclodextrin

Cyclodextrins are hexasaccharides that are natural starch-conversion products. Industrially, they are manufactured by enzymatic degradation of vegetable raw materials.

Alpha-cyclodextrin has the chemical formula C₃₆H₆₀O₃₀. It is said to lower blood pressure, cholesterol, and glucose levels. It is primarily used as a dietary supplement for fiber.

Beta-cyclodextrin has the chemical formula C₄₂H₇₀O₃₅. It is used in a large variety of industries primarily as a complexing agent.  It’s most notable uses are in medicine as sustained release drugs carrier and to improve stability in drugs.

Gamma-cyclodextrin has the chemical formula C₄₈H₈₀O₄₀. It is used as a complexing agent due to having the largest cavity size between the natural cyclodextrin. It has use across a variety of industries from food, to medicine, to textiles, and cosmetics.