HPLC Method For Analysis of Cyclodextrins on Primesep S2

Separation type: Liquid Chromatography HILIC

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High Performance Liquid Chromatography (HPLC) Method for Analysis of B-Cyclodextrin, G-Cyclodextrin and A-Cyclodextrin


Column Primesep S2, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN- 70%
Buffer AmFm pH 3.0- 20 mm
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Sugar
Analyzing Compounds B-Cyclodextrin, A-Cyclodextrin, G-Cyclodextrin


Application Column

Primesep S2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.