Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Ferrozine check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was diluted in water.

HPLC Method for Ferrozine on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ferrozine

Ferrozine is a common reagent and indicator used for analyzing and detecting iron. Multi-charged molecules, such as Ferrozine, generally have a tendency to exhibit poor peak shape on reverse-phase HPLC columns, where they show significant tailing.

This compound can be retained with great peak shape on a Newcrom BH mixed-mode column. Ferrozine can be measured at low UV. Using a Newcrom BH mixed-mode column and a mobile phase consisting of methanol (MeOH) and water with perchloric acid (HCLO4) buffer, Ferrozine can be retained and UV detected at 280 nm with precise resolution.