| CAS Number | 28048-33-1 |
|---|---|
| Molecular Formula | C20H12N4Na2O6S2 |
| Molecular Weight | 514.4 |
| InChI Key | DDIHLFCSEHAOJZ-UHFFFAOYSA-L |
| Synonyms |
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Applications:
HPLC Method of Ferrozine on Newcrom BH Column
October 11, 2021
HPLC Method for Ferrozine on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ferrozine
Ferrozine is a common reagent and indicator used for analyzing and detecting iron. Multi-charged molecules, such as Ferrozine, generally have a tendency to exhibit poor peak shape on reverse-phase HPLC columns, where they show significant tailing.
This compound can be retained with great peak shape on a Newcrom BH mixed-mode column. Ferrozine can be measured at low UV. Using a Newcrom BH mixed-mode column and a mobile phase consisting of methanol (MeOH) and water with perchloric acid (HCLO4) buffer, Ferrozine can be retained and UV detected at 280 nm with precise resolution.
| Column | Newcrom BH, 2.1 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeOH/H2O – 30/70% |
| Buffer | HClO4 – 0.3% |
| Flow Rate | 0.2 ml/min |
| Detection | UV 280 nm |
| Class of Compounds | Indicator |
| Analyzing Compounds | Ferrozine |
Application Column
Newcrom BH
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
