HPLC Method of Ferrozine on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Ferrozine

Ferrozine is a common reagent and indicator used for analyzing and detecting iron. Multi-charged molecules, such as Ferrozine, generally have a tendency to exhibit poor peak shape on reverse-phase HPLC columns, where they show significant tailing. This compound can be retained with great peak shape on a Newcrom BH mixed-mode column. Ferrozine can be measured at low UV. Using a Newcrom BH mixed-mode column and a mobile phase consisting of methanol (MeOH) and water with perchloric acid (HCLO4) buffer, Ferrozine can be retained and UV detected at 280 nm with precise resolution.

Condition

Column Newcrom BH, 2.1×100 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 30/70%
Buffer HClO4 – 0.3%
Flow Rate 0.2 ml/min
Detection UV 280  nm

Description

Class of Compounds
Analyzing Compounds Ferrozine

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Ferrozine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.