Epibatidine

CAS Number140111-52-0
Molecular FormulaC11H13ClN2
Molecular Weight208.69
InChI KeyNLPRAJRHRHZCQQ-IVZWLZJFSA-N
LogP2.2
Synonyms
  • Epibatidine
  • 140111-52-0
  • CMI-488
  • (1R,2R,4S)-Epibatidine
  • M6K314F1XX
  • DTXSID00894067
  • 7-Azabicyclo(2.2.1)heptane, 2-(6-chloro-3-pyridinyl)-, exo-
  • 7-Azabicyclo(2.2.1)heptane, 2-(6-chloro-3-pyridinyl)-, (1R,2R,4S)-
  • 7-Azabicyclo[2.2.1]heptane, 2-(6-chloro-3-pyridinyl)-, (1R,2R,4S)-
  • DTXSID30861409
  • RefChem:590908
  • DTXCID20197325
  • DTXCID001324110
  • 3-(6-chloropyridin-3-yl)-7-azabicyclo(2.2.1)heptane
  • 634-286-9
  • (+)-Epibatidine
  • (1S,2S,4R)-2-(6-chloropyridin-3-yl)-7-azabicyclo(2.2.1)heptane
  • 2-(6-Chloropyridin-3-yl)-7-azabicyclo(2.2.1)heptane
  • (1R,2R,4S)-2-(6-Chloropyridin-3-yl)-7-azabicyclo[2.2.1]heptane
  • (-)-Epibatidine
  • CHEMBL298826
  • EPJ
  • UNII-M6K314F1XX
  • EPIBATIDINE [MI]
  • orb2945537
  • SCHEMBL2500147
  • 2-(6-Chloro-pyridin-3-yl)-7-aza-bicyclo[2.2.1]heptane (epibatidine)
  • CMI-477
  • CMI-545
  • (2R)-2-(6-CHLOROPYRIDIN-3-YL)-7-AZABICYCLO[2.2.1]HEPTANE
  • BDBM50143314
  • DB07720
  • ALKALOID 208/210 FROM DENDROBATES
  • HY-169742
  • NS00068280
  • (1R,2R,4S)-2-(6-Chloro-pyridin-3-yl)-7-aza-bicyclo[2.2.1]heptane
  • (1R-EXO)-2-(6-CHLORO-3-PYRIDINYL)-7-AZABICYCLO(2.2.1)HEPTANE
  • 7-AZABICYCLO(2.2.1)HEPTANE, 2-(6-CHLORO-3-PYRIDINYL)-, (1R-EXO)-

Applications:

HPLC Method for Analysis of Epibatidine on Primesep 200 Column

February 19, 2026

HPLC Method for Epibatidine on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Epibatidine

Epibatidine is an alkaloid with the chemical formula C11H13ClN3. It is secreted by the Ecuadorian frog commonly known as Anthony’s Poison Arrow frog from the genus of poison dart frogs, Ameerega. It’s existence in nature is still debated due to limited samples that were collected by John W. Daly, and even fewer samples that were still left when high resolution spectrometry was popularized and used on the compound. As a poison, Epibatidine causes full body numbness which can progress into full body paralysis and respiratory arrest. Due to it’s high toxicity, it is not used as a drug, despite the analgesic effect. You can find detailed UV spectra of Epibatidine and information about its various lambda maxima by visiting the following link.

Epibatidine can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 30/70%
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/m
DetectionUV 215nm
Class of Compounds
 Poison, Alkaloid, Pyridine derivative, Azabicyclic compound, Organochlorine compound
Analyzing CompoundsEpibatidine

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Epibatidine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Uv-Vis Spectrum of Epibatidine

February 19, 2026

Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Epibatidine check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Epibatidine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.