Determination Pantoprazole on Obelisc R Column

HPLC Method for Pantoprazole, Pantoprazole sodium on Obelisc R by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pantoprazole, Pantoprazole sodium.

Pantoprazole is a proton pump inhibitor (PPI) with C16H15F2N3O4S molecular formula. It is used to decrease stomach acids amounts. It does that through binding to proton pumps in the stomach’s lining, therefore preventing the production of stomach acid.

Pantoprazole, Pantoprazole sodium can be retained on an Obelisc R column, which has both positive and negative ion-pairs embedded in the stationary phase, allowing for the fine tuning and separation of a wide range of compounds with different ionic properties. Pantoprazole can be determined isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer and detected by UV at 290nm, ELSD, CAD or LC/MS.

Condition
Column Obelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN -35%
Buffer Ammonium Acetate  pH 5.5 – 10 mM
Flow Rate 1.0 ml/min
Detection UV 290 nm

 

Description
Class of Compounds
  Drug
Analyzing Compounds Pantoprazole, Pantoprazole sodium

 

Application Column

Obelisc R

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Pantoprazole
Pantoprazole sodium
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.