| CAS Number | 14698-29-4 |
|---|---|
| Molecular Formula | C13H11NO5 |
| Molecular Weight | 261.234 |
| InChI Key | KYGZCKSPAKDVKC-UHFFFAOYSA-N |
| LogP | 0.94 |
| Synonyms |
|
Applications:
UV-Vis Spectrum of Oxolinic acid
January 5, 2026
If you are looking for optimized HPLC method to analyze Oxolinic acid check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Separation of a Complex of Quinolone Antibiotics
May 27, 2015

Quinolones are a group of synthetic broad-spectrum antibiotics with a wide variety of applications in medicine and veterinary sciences. Some quinolones have been found in food products including fish and shrimp. Primesep 100 was used to separate a mixture of quinolones and achieve baseline separation. Retention was controlled by adjusting acetonitrile in the mobile phase.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Quinolone Antibiotics, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Oxolinic Acid, Flumequine, Ciprofloxacin, Enrofloxacin, Pefloxacin |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsEnrofloxacin
Flumequine
Oxolinic acid
Pefloxacin

