Quinolones are a group of synthetic broad-spectrum antibiotics with a wide variety of applications in medicine and veterinary sciences. Some quinolones have been found in food products including fish and shrimp. Primesep 100 was used to separate a mixture of quinolones and achieve baseline separation. Retention was controlled by adjusting acetonitrile in the mobile phase.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Drug, Acid, Quinolone Antibiotics, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Oxolinic Acid, Flumequine, Ciprofloxacin, Enrofloxacin, Pefloxacin |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsEnrofloxacin
Flumequine
Oxolinic acid
Pefloxacin
