Melamine

Melamine (1,3,5-triazine-2,4,6-triamine) structural formula

CAS Number108-78-1
Molecular FormulaC3H6N6
Molecular Weight126.124
InChI KeyJDSHMPZPIAZGSV-UHFFFAOYSA-N
LogP-1.37
Synonyms
  • Melamine
  • 1,3,5-Triazine-2,4,6-triamine
  • 108-78-1
  • 1,3,5-Triazine-2,4,6-triamine
  • 4-26-00-01253
  • 1,3,5-Triazine, 2,4,6-triamine
  • 1,3,5-Triazine-2,4,6(1H,3H,5H)-triimine
  • 2,4,6-Triamino-1,2,3-triazine
  • 2,4,6-Triamino-1,3,5-triazine
  • 2,4,6-Triamino-s-triazine
  • 2,4,6-Triaminotriazine
  • ADK Stab ZS 27
  • Cyanuramide
  • Cyanurotriamide
  • Cyanurotriamine
  • Flammex MEL
  • Isomelamine
  • Melamin
  • melamina
  • NSC 2130
  • Pluragard
  • Pluragard C 133
  • Spinflam ML 94M
  • s-Triazine, 4,6-diamino-1,2-dihydro-2-imino-
  • s-Triazinetriamine
  • Teoharn
  • Theoharn
  • Triamino-s-triazine
  • Triaminotriazine
  • TRIAZINE (1,3,5), 2,4,6-TRIAMINO-
  • UREA MELAMINE
  • Virset 656-4
  • Yukamelamine
  • BRN 0124341
  • EINECS 203-615-4
  • Hicophor PR
  • NCI-C50715
  • s-Triazine, 2,4,6-triamino-
  • UNII-N3GP2YSD88
  • Cyanuric triamide
  • sym-triaminotriazine
  • 1228929-27-8
  • 130392-03-9
  • 169314-62-9
  • 504-18-7
  • 65544-34-5
  • 67757-43-1
  • 68379-55-5
  • 70371-19-6
  • 94977-27-2

Applications:

HPLC Separation of Acrylamide and Melamine on Primesep 200 Column

September 4, 2020


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Melamine is a nitrogen-based organic compound that was first synthesized by the German chemist Justus von Liebig in 1834. Melamine is a decorative coating that is resistant to water and mechanical damage.

Melamine is used for:

  • lamination of particle boards and plywood;
  • manufacture of paints and varnishes;
  • production of decorative paper-laminated plastics and plastics;
  • manufacture of dishes.

We were able to separate Melamine from Acrylamide in HPLC on Primesep 200 column using a mobile phase of water with Formic Acid buffer. This separation can be monitored with UV, ELSD, CAD detection techniques.

Condition

Column Primesep 200, 4.6×150 mm, 3 µm, 100A
Mobile Phase H2O – 99%
Buffer Formic Acid – 1%
Flow Rate 1.0 ml/min
Detection UV 240 nm

 

Description

Class of Compounds Amide, Amine
Analyzing Compounds Acrylamide,  Melamine

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Acrylamide
Melamine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for the Simultaneous Separation of Melamine and Cyanuric Acid on an Obelisc R Column

July 8, 2011

chr_286.gif

Melamine is an organic basic compound that is derived from cyanamide. It contains 66% nitrogen by mass. Addition of melamine is used to increase protein count in milk products. Cyanuric acid is a trimer of cyanic acid. Melamine and cyanuric acid can form a insoluble complex. Both compounds has been linked as additives to modify nitrogen count in products. Cyanuric acid is a slightly acidic hydrophilic compound, while melamine is a basic hydrophilic compound. Both compounds were separated on Obelisc R reversed-phase ion-exchange column with LC/Ms compatible mobile phase. Method can be used for generic analysis of basic and acidic compounds with LC/MS compatible conditions.

Condition

Column Obelisc R, 4.6 x 150 mm, 5 µm, 100 A
Mobile Phase MeCN
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD 50C

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Description

Class of Compounds
 Acid,  Hydrophobic, Ionizable
Analyzing Compounds Melamine,  Cyanuric Acid

Application Column

Obelisc R

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Cyanuric Acid
Melamine

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Application for Determination of Melamine in Baby Formula

September 24, 2010

HPLC Method for Analysis of Melamine on Primesep 200 by SIELC Technologies

HPLC Application for Determination of Melamine in Baby Formula by SIELC Technologies

Melamine (1,3,5-triazine-2,4,6-triamine) is an organic base with pKa of 5. It is only slightly soluble in water. Melamine is a trimer of cyanamide. Melamine is a metabolite of pesticide cyromazine. Cyromazine is converted to melamine in plants. Melamine is used in production of plastics. In 2007 it was determined that melamine caused hundreds of pet deaths due to pet food contamination. Prior to these reports, melamine had been regarded as non-toxic or minimally toxic. However, because of the presence of the melamine in wheat gluten added to mass-produced dog and cat foods, it is the most likely cause. There is some data proving that melamine and cyanuric acid form a complex that show much higher toxicity.
It was found recently that melamine was added to various products (including milk formulas) to artificially inflate the reading for protein levels. Formula milk has not, until now, been tested for melamine, because regulators did not suspect this ingredient might be added. Indeed, it is this high nitrogen level – 66% nitrogen by mass – in melamine that gives it the analytical characteristics of protein molecules. A simple and fast HPLC method for determination of melamine is developed. Melamine is well retained on short (50 mm) Primesep columns. The retention is attributed to reverse phase and cation-exchange mechanism. Method uses acetonitrile-water based mobile phase with formic acid as an acidic modifier and Primesep 200 column. Method can be used in determination of melamine in food, milk, baby formulas, pet food, and other products by HPLC by direct injection or with preliminary protein clean up by ACN precipitation and filtration. Both MS (mass spectroscopy) detection and UV (ultra violet) detection at 240 nm can be effectively used to quantitate the presence and amounts of melamine with this method.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Melamine

ColumnPrimesep 200, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 10%
BufferFormic Acid – 0.25%
Flow Rate1.0 ml/min
DetectionUV 240nm
Class of Compounds
Hydrophobic, Ionizable
Analyzing CompoundsMelamine

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Melamine

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for the Simultaneous Separation of Melamine and Cyanuric Acid on an Obelisc N Column

June 11, 2004

chr_286.gif

Melamine is an organic basic compound that is derived from cyanamide. It contains 66% nitrogen by mass. Addition of melamine is used to increase protein count in milk products. Cyanuric acid is a trimer of cyanic acid. Melamine and cyanuric acid can form a insoluble complex. Both compounds has been linked as additives to modify nitrogen count in products. Cyanuric acid is a slightly acidic hydrophilic compound, while melamine is a basic hydrophilic compound. Both compounds were separated on Obelisc R reversed-phase ion-exchange column with LC/Ms compatible mobile phase. Method can be used for generic analysis of basic and acidic compounds with LC/MS compatible conditions.

Condition

Column Obelisc N, 4.6 x 150 mm, 5 µm, 100 A
Mobile Phase MeCN
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD 50C

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Description

Class of Compounds
 Acid,  Hydrophobic, Ionizable
Analyzing Compounds Melamine,  Cyanuric Acid

Application Column

Obelisc N

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Cyanuric Acid
Melamine

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.