HPLC Separation of Acrylamide and Melamine on Primesep 200 Column

HPLC Method for Acrylamide, Melamine on Primesep 200 by SIELC Technologies

 

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Acrylamide, Melamine.

Melamine is a nitrogen-based organic compound that was first synthesized by the German chemist Justus von Liebig in 1834. Melamine is a decorative coating that is resistant to water and mechanical damage.

Melamine is used for:

  • lamination of particle boards and plywood;
  • manufacture of paints and varnishes;
  • production of decorative paper-laminated plastics and plastics;
  • manufacture of dishes.

We were able to separate Melamine from Acrylamide in HPLC on Primesep 200 column using a mobile phase of water with Formic Acid buffer. This separation can be monitored with UV, ELSD, CAD detection techniques.

Condition

Column Primesep 200, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile Phase H2O – 99%
Buffer Formic Acid – 1%
Flow Rate 1.0 ml/min
Detection UV 240 nm

 

Description

Class of Compounds Amide, Amine
Analyzing Compounds Acrylamide, Melamine

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Acrylamide
Melamine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.