HPLC Separation of Acrylamide and Melamine on Primesep 200 Column

HPLC.cloud View on hplc.cloud   Melamine is a nitrogen-based organic compound that was first synthesized by the German chemist Justus von Liebig in 1834. Melamine is a decorative coating that is resistant to water and mechanical damage. Melamine is used for:
  • lamination of particle boards and plywood;
  • manufacture of paints and varnishes;
  • production of decorative paper-laminated plastics and plastics;
  • manufacture of dishes.
We were able to separate Melamine from Acrylamide in HPLC on Primesep 200 column using a mobile phase of water with Formic Acid buffer. This separation can be monitored with UV, ELSD, CAD detection techniques.


Column Primesep 200, 4.6x150 mm, 3 µm, 100A
Mobile Phase H2O - 99%
Buffer Formic Acid - 1%
Flow Rate 1.0 ml/min
Detection UV 240 nm


Class of Compounds Amide, Amine
Analyzing Compounds Acrylamide,  Melamine

Application Analytes:
Melamine (1,3,5-triazine-2,4,6-triamine)