| CAS Number | 1032-65-1 |
|---|---|
| Molecular Formula | C9H14N3O7P |
| Molecular Weight | 307.2 |
| InChI Key | NCMVOABPESMRCP-SHYZEUOFSA-N |
| LogP | -3.4 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Deoxycytidine monophosphate
February 2, 2026
If you are looking for optimized HPLC method to analyze 2'-Deoxycytidine 5'-Monophosphate check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Separation of Cytidine-5′-monophosphate (CMP) and 2′-Deoxycytidine 5′-Monophosphate (dCMP) on Newcrom B Column
October 25, 2019
HPLC Method for 2'-Deoxycytidine 5'-Monophosphate, Cytidine Monophosphate on Newcrom B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2'-Deoxycytidine 5'-Monophosphate, Cytidine Monophosphate .
Cytidine-5′-monophosphate is a nucleoside monophosphate with C9H14N3O8P chemical formula. It is a fundamental building block for RNA and DNA as well as an intermediate in phospholipid synthesis.
2′-Deoxycytidine 5′-Monophosphate is a deoxyribonucleotide with C9H14N3O7P chemical formula. It is crucial when it comes to DNA synthesis. dCMP is often used in genetic research, biotechnology, and development of pharmaceuticals with a focus on antiviral and anticancer drugs.
2'-Deoxycytidine 5'-Monophosphate, Cytidine Monophosphate can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeOH/H2O – 80/20% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | 2'-Deoxycytidine 5'-Monophosphate, Cytidine Monophosphate |
Application Column
Newcrom B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Cytidine Monophosphate
