HPLC Separation of Cytidine-5′-monophosphate (CMP) and 2′-Deoxycytidine 5′-Monophosphate (dCMP) on Newcrom B Column

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HPLC Separation of CMP and dCMP on Newcrom B Column_1193


Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 80/20%
Buffer H3PO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 270nm


Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds 2′-Deoxycytidine 5′-Monophosphate



Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
2′-Deoxycytidine 5′-Monophosphate
Cytidine Monophosphate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.