HPLC Separation of Cytidine-5′-monophosphate (CMP) and 2′-Deoxycytidine 5′-Monophosphate (dCMP) on Newcrom B Column

HPLC Method for 2'-Deoxycytidine 5'-Monophosphate, Cytidine Monophosphate on Newcrom B by SIELC Technologies

HPLC Separation of CMP and dCMP on Newcrom B Column_1193

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2'-Deoxycytidine 5'-Monophosphate, Cytidine Monophosphate .

Cytidine-5′-monophosphate is a nucleoside monophosphate with C9H14N3O8P chemical formula. It is a fundamental building block for RNA and DNA as well as an intermediate in phospholipid synthesis.

2′-Deoxycytidine 5′-Monophosphate is a deoxyribonucleotide with C9H14N3O7P chemical formula. It is crucial when it comes to DNA synthesis. dCMP is often used in genetic research, biotechnology, and development of pharmaceuticals with a focus on antiviral and anticancer drugs.

2'-Deoxycytidine 5'-Monophosphate, Cytidine Monophosphate can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.

Condition

Column Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeOH/H2O – 80/20%
Buffer H3PO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 270nm

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds 2'-Deoxycytidine 5'-Monophosphate, Cytidine Monophosphate

 

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
2′-Deoxycytidine 5′-Monophosphate
Cytidine Monophosphate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.