Chlorthalidone

Chlorthalidone structural formula

CAS Number77-36-1
Molecular FormulaC14H11ClN2O4S
Molecular Weight338.761
InChI KeyJIVPVXMEBJLZRO-UHFFFAOYSA-N
LogP0.85
Synonyms
  • Chlorthalidone
  • 2-Chloro-5-(1-hydroxy-3-oxo-2,3-dihydro-1H-isoindol-1-yl)benzene-1-sulfonamide
  • Benzenesulfonamide, 2-chloro-5-(2,3-dihydro-1-hydroxy-3-oxo-1H-isoindol-1-yl)-
  • 77-36-1
  • 5-22-07-00602
  • Benzenesulfonamide, 2-chloro-5-(2,3-dihydro-1-hydroxy-3-oxo-1H-isoindol-1-yl)-
  • (.+-.)-Chlorthalidone
  • (.+-.)-Hygroton
  • 1-keto-3-(3'-Sulfamyl-4'-chlorophenyl)-3-hydroxyisoindoline
  • 1-Oxo-3-(3-sulfamyl-4-chlorophenyl)-3-hydroxyisoindoline
  • 1-Oxo-3-(3-sulfamyl-4-chlorophenyl)-3-hydroxy-isoindolinum
  • 1-Oxo-3-(4'-chloro-3'-sulphamoylphenyl)-3-hydroxyisoindoline
  • 2-Chloro-5-(1-hydroxy-3-oxo-1-isoindolinyl)benzenesulfonamide
  • 2-Chloro-5-(3-hydroxy-1-oxoisoindol-3-yl)benzenesulfonamide
  • 3-(4'-Chloro-3'-sulfamoylphenyl)-3-hydroxyphthalimidine
  • 3-Hydroxy-3-[4-chloro-3-sulfamylphenyl]phthalimidine
  • Benzenesulfonamide, 2-chloro-5-(1-hydroxy-3-oxo-1-isoindolinyl)-
  • Chlorothalidone
  • Chlortalidon
  • Chlortalidone
  • Chlorthalidon
  • clortalidona
  • Demi-Regroton
  • Hygroton
  • Igroton
  • Natriuran
  • NSC 69200
  • Oxodolin
  • Phthalamudine
  • Racemic chlorthalidone
  • Saluretin
  • Thalitone
  • Zambesil
  • BRN 0312295
  • Chlorphthalidone
  • EINECS 201-022-5
  • Hydro-Long
  • (+-)-Chlorthalidone
  • (+-)-Hygroton
  • Famolin
  • Chlortalidonum
  • Clortalidone
  • UNII-Q0MQD1073Q
  • 2-chloro-5-(2,3-dihydro-1-hydroxy-3-oxo-1H-isoindol-1-yl)benzenesulfonamide
  • 3-hydroxy-3-(4-chloro-3-sulfamylphenyl)phthalimidine
  • chlorphthalidolone
  • phthalamodine

Applications:

HPLC Chiral Separation of Chlorthalidone Enantiomers on Cyralis CL Column

September 29, 2026

HPLC Chiral Method for Analysis of Chlorthalidone Enantiomers on Cyralis CL Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Chlorthalidone.

Chlorthalidone, also known as Chloratalidone, is a thiazide-like diuretic drug with the chemical formula C14H11ClN2O4S. It is used primarily in treatment of high blood pressure, swelling caused by heart and liver failure, renal tubular acidosis, and more. Less commonly, itis used to treat calcium-based kidney stones. It works through reducing the reabsorption of sodium and chloride by inhibiting the Na+/Cl– symporter in the apical membrane of distal convoluted tubule cells in the kidney. It is banned in cricket, wrestling, and boxing.

This application describes a normal-phase chiral HPLC method for the separation of Chlorthalidone enantiomers using SIELC’s Cyralis CL chiral column. Cyralis CL is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Chlorthalidone can be retained and analyzed using the Cyralis CL stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of hexane, ethanol, and TFA. Detection is performed using UV.

Condition

ColumnCyralis CL, 4.6 x 100 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/TFA– 50/50/0.1%
Flow Rate1.0 mL/min
DetectionUV 220 nm
Resolution2.72

Description

Class of CompoundsDrug
Analyzing CompoundsChlorthalidone

Application Column

Cyralis CL

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Chlorthalidone
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Chlorthalidone on Newcrom R1 HPLC column

February 16, 2018
Separation of Chlorthalidone on Newcrom C18 HPLC column

Chlorthalidone can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Chlorthalidone
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.