HPLC Chiral Separation of Chlorthalidone Enantiomers on Cyralis CL Column

HPLC Chiral Method for Analysis of Chlorthalidone Enantiomers on Cyralis CL Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Chlorthalidone.

Chlorthalidone, also known as Chloratalidone, is a thiazide-like diuretic drug with the chemical formula C14H11ClN2O4S. It is used primarily in treatment of high blood pressure, swelling caused by heart and liver failure, renal tubular acidosis, and more. Less commonly, itis used to treat calcium-based kidney stones. It works through reducing the reabsorption of sodium and chloride by inhibiting the Na+/Cl– symporter in the apical membrane of distal convoluted tubule cells in the kidney. It is banned in cricket, wrestling, and boxing.

This application describes a normal-phase chiral HPLC method for the separation of Chlorthalidone enantiomers using SIELC’s Cyralis CL chiral column. Cyralis CL is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Chlorthalidone can be retained and analyzed using the Cyralis CL stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of hexane, ethanol, and TFA. Detection is performed using UV.

Condition

ColumnCyralis CL, 4.6 x 100 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/TFA– 50/50/0.1%
Flow Rate1.0 mL/min
DetectionUV 220 nm
Resolution2.72

Description

Class of CompoundsDrug
Analyzing CompoundsChlorthalidone

Application Column

Cyralis CL

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Chlorthalidone
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.