Bicine

Bicine structural formula

CAS Number150-25-4
Molecular FormulaC6H13NO4
Molecular Weight163.174
InChI KeyFSVCELGFZIQNCK-UHFFFAOYSA-N
LogP-1.51
Synonyms
  • Bicine
  • N,N-Bis(2-hydroxyethyl)glycine
  • Glycine, N,N-bis(2-hydroxyethyl)-
  • 150-25-4
  • 4-04-00-02390
  • N,N-Bis(2-hydroxyethyl)aminoacetic acid
  • N,N-Bis(beta-hydroxyethyl)glycine
  • Bis(2-Hydroxyethyl)glycine
  • BRN 1769362
  • Diethanol glycine
  • Diethylolglycine
  • N,N-Dihydroxyethyl glycine
  • Dihydroxyethylglycine
  • N,N-Dihydroxyethylglycine
  • N,N-Di(2-hydroxyethyl)glycine
  • EINECS 205-755-1
  • Fe-3-Specific
  • N,N-(2-Hydroxyethyl)glycine
  • NSC 7342
  • UNII-1J484QFI1O

Applications:

HPLC Method for Analysis of Bicine on Primesep 100 Column

May 4, 2022

Separation type: Liquid Chromatography Mixed-mode

 


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Bicine

 

Bicine is a common organic buffering agent used in low temperature conditions. It is one of many buffering agents known as “Good’s buffers,” and like many of them, Bicine is zwitterionic. Bicine can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry

 

Condition
Column Promesep 100, 4.6×150 mm,  100A
Mobile Phase MeCN – 20%
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description
Class of Compounds Amino acid
Analyzing Compounds Bicine

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Bicine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Bicine on Newcrom R1 HPLC column

February 16, 2018
Separation of Bicine on Newcrom C18 HPLC column

Bicine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Bicine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.