HPLC Method for Analysis of Bicine on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode

 


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Bicine

 

Bicine is a common organic buffering agent used in low temperature conditions. It is one of many buffering agents known as “Good’s buffers,” and like many of them, Bicine is zwitterionic. Bicine can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry

 

Condition
Column Promesep 100, 4.6×150 mm,  100A
Mobile Phase MeCN – 20%
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description
Class of Compounds Amino acid
Analyzing Compounds Bicine

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Bicine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.