Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bicine
Bicine is a common organic buffering agent used in low temperature conditions. It is one of many buffering agents known as “Good’s buffers,” and like many of them, Bicine is zwitterionic. Bicine can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 20% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Sample | 8 mg/ml in H2O |
| LOD* | 3 ppm |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Amino acid |
| Analyzing Compounds | Bicine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
