HPLC Method for Bicine on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bicine.
Bicine is a common organic buffering agent wit the chemical formula C6H13NO4. It is one of many buffering agents known as “Good’s buffers,” and like many of them, Bicine is zwitterionic. It is used in low temperature conditions due to it’s stable pH and high solubility even at 0oC.
Bicine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 20% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Sample | 8 mg/ml in H2O |
| LOD* | 3 ppm |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Class of Compounds | Amino acid |
| Analyzing Compounds | Bicine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
