Toluidines

Synonyms

    Applications:

    HPLC Separation of Toluidine Isomers in Cation-Exchange Mode

    July 14, 2011

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    Isomers of toluidine were successfully retained and separated in cation-exchange mode on a Primesep S2 column. Primesep S2 column is a HILIC/cation-exchange column that can be operated in HILIC, cation-exchange, and anion-exclusion modes. Method and column are compatible with ELSD/LC/MS and UV and can be used for routine analysis of polar molecules.

    Application Column

    Primesep S2

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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    Application Analytes:
    Toluidines
    m-Toluidine
    o-Toluidine
    p-Toluidine

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Method for Separation of Toluidine Isomers on Primesep 200 Coulmn

    December 31, 2005

    Toluidine isomers are based on toluene and differ whether the primary amine is ortho, meta or para to the methyl group. A Primesep 200 column with an ammonium formate buffer or a Primesep 100 column with sodium phosphate baseline resolves the three regioisomers. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), ammonium formate, or sodium phosphate and ultraviolet (UV) detection at 250 nm.

    Application Column

    Primesep 200

    Column Diameter: 4.6 mm
    Column Length: 150 mm
    Particle Size: 5 µm
    Pore Size: 100 A

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    Application Analytes:
    Toluidines
    m-Toluidine
    o-Toluidine
    p-Toluidine

    Application Detection:
    ELSD Detection
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.