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Applications:
HPLC Analysis of Hop Extract (Beer Brewing)
October 14, 2010
Hop extract is a complex mixture of alpha and beta acids, essential oils, tannins, and waxes. Components of the hop extract were separated by mixed-mode HPLC on a Primesep SB column. Compounds on this column are retained by combination of reversed-phase and anion-exchange mechanism. At higher pH (3-5), alpha and beta acids are ionized. Retention time can be controlled by amount of acetonitrile, buffer pH, and buffer concentration. Primesep SB column is fully ionized within operational range of the column.
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsTetrahydro-Iso-Alpha Acids
