HPLC Analysis of Hop Extract (Beer Brewing)


Hop extract is a complex mixture of alpha and beta acids, essential oils, tannins, and waxes. Components of the hop extract were separated by mixed-mode HPLC on a Primesep SB column. Compounds on this column are retained by combination of reversed-phase and anion-exchange mechanism. At higher pH (3-5), alpha and beta acids are ionized. Retention time can be controlled by amount of acetonitrile, buffer pH, and buffer concentration. Primesep SB column is fully ionized within operational range of the column.

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Iso-Alpha Acids
Tetrahydro-Iso-Alpha Acids
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.