| CAS Number | 155213-67-5 |
|---|---|
| Molecular Formula | C37H48N6O5S2 |
| Molecular Weight | 720.951 |
| InChI Key | NCDNCNXCDXHOMX-XGKFQTDJSA-N |
| LogP | 4.63 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Nirmatrelvir and Ritonavir in Paxlovid Tablets on Primesep P column
June 15, 2022
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Nirmatrelvir and Ritonavir
| Column | Promesep P, 3.2×100 mm, 100A |
| Mobile Phase | Gradient MeCN – 40-75% |
| Buffer | HClO4 – 0.5% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 210nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Nirmatrelvir, Ritonavir |
Application Column
Primesep P
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsRitonavir
HPLC Method for Analysis of Nirmatrelvir and Ritonavir in Paxlovid Tablets on Primesep 100 Column
May 5, 2022
HPLC Method for Ritonavir, Nirmatrelvir on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ritonavir, Nirmatrelvir.
Nirmatrelvir is an antiviral medication with the chemical formula C23H32F3N5O4. It is a part of a medical combination used to treat COVID-19. It works through inhibiting 3C-like protease. From November 2021, Nirmatrelvir has been allowed to be manufactured and sold in over 95 countries to ensure greater access to the global population.
Ritonavir is an antiretroviral medication with the chemical formula C37H48N6O5S2. When used alone, it is used to treat HIV/AIDS. In combination with other drugs, especially potease inhibitors, it is used to treat Hepatitis C and COVID-19.
Ritonavir, Nirmatrelvir can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Sulfuric Acid buffer. Detection is performed using UV.
| Column | Primesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 50-70% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 210nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Ritonavir, Nirmatrelvir |
Application Column
Primesep 100
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Ritonavir
HPLC Separation of Ritonavir and Ribavirin
August 22, 2008
Separation type: Liquid Chromatography HILIC
Ritonavir and Ribavarin are two drugs which are very different in their properties for HPLC separation. Very hydrophobic and very hydrophilic drugs are separated on Primesep N using Hydrophilic Interaction Chromatography (HILIC). Ritonavir is an antiretroviral drug from the protease inhibitor class used to treat HIV infection and AIDS. Ritonavir is very hydrophobic and non-ionizable and retains very long in reverse phase chromatography. Ribavirin is an anti-viral drug which is active against a number of DNA and RNA viruses. It is a member of the nucleoside antimetabolite drug, which makes it very hydrophilic.
| Column | Primesep N, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 97/3% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm, MS- compatible mobile phase |
| Class of Compounds | Drug |
| Analyzing Compounds | Ritonavir, Ribavirin |
Application Column
Primesep N
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Ritonavir
