Separation type: Liquid Chromatography HILIC
Ritonavir and Ribavarin are two drugs which are very different in their properties for HPLC separation. Very hydrophobic and very hydrophilic drugs are separated on Primesep N using Hydrophilic Interaction Chromatography (HILIC). Ritonavir is an antiretroviral drug from the protease inhibitor class used to treat HIV infection and AIDS. Ritonavir is very hydrophobic and non-ionizable and retains very long in reverse phase chromatography. Ribavirin is an anti-viral drug which is active against a number of DNA and RNA viruses. It is a member of the nucleoside antimetabolite drug, which makes it very hydrophilic.
| Column | Primesep N, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 97/3% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm, MS- compatible mobile phase |
| Class of Compounds | Drug |
| Analyzing Compounds | Ritonavir, Ribavirin |
Application Column
Primesep N
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
RibavirinRitonavir
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
