Dinitrophenylacetic Acid

Dinitrophenylacetic Acid

CAS Number643-43-6
Molecular FormulaC8H6N2O6
Molecular Weight226.144
InChI KeyKCNISYPADDTFDO-UHFFFAOYSA-N
LogP1.1
Synonyms
  • 2,4-Dinitrophenylacetic acid
  • 2-(2,4-dinitrophenyl)acetic acid
  • (2,4-Dinitrophenyl)acetic acid
  • NSC6225
  • Benzeneacetic acid, 2,4-dinitro-
  • NSC-6225
  • SBB028551
  • ST50308304
  • ACMC-209nlg
  • AC1L5ADM
  • AC1Q5VIW
  • 2,4-dimtrophenylacetic acid
  • NCIStruc1_000234
  • NCIStruc2_000110
  • 2,4-Dinitrobenzeneacetic acid
  • Benzeneacetic acid,4-dinitro-
  • SCHEMBL865986
  • 2,4-DINITROPHENOXAZINE
  • Acetic acid,4-dinitrophenyl)-
  • AC1Q74V0
  • CHEMBL1741495
  • CTK5C1109
  • Benzeneacetic acid,2,4-dinitro-
  • NCI6225
  • KCNISYPADDTFDO-UHFFFAOYSA-N
  • MolPort-001-769-147
  • (2,4-dinitro-phenyl)-acetic acid
  • 2,4-DINITROPHENYLACETICACID
  • KS-000048TP

Applications:

Uv-Vis Spectrum of 2,4-Dinitrophenylacetic Acid

February 13, 2026
Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Dinitrophenylacetic Acid check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

 

 

 

 

 

 

 

 

 

 

 

 

Application Analytes:
Dinitrophenylacetic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Guanidine and Benzoic Acids on Primesep 100 Column

October 4, 2007

Condition
Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 230 nm, ELSD

 

Description
Class of Compounds
 Acid, Hydrophilic, Ionizable
Analyzing Compounds Guanidine, Benzoic acid, Dinitrophenylacetic Acid

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Benzoic Acid
Dinitrophenylacetic Acid
Guanidine
Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.