HPLC Separation of Guanidine and Benzoic Acids on Primesep 100 Column


Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 230 nm, ELSD



Class of Compounds
Acid, Hydrophilic, Ionizable
Analyzing Compounds Guanidine, Benzoic acid, Dinitrophenylacetic Acid


SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep 100

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
Benzoic Acid
Dinitrophenylacetic Acid

Application Detection:
UV Detection
ELSD/MS Detection