| CAS Number | 610-27-5 |
|---|---|
| Molecular Formula | C8H5NO6 |
| Molecular Weight | 211.129 |
| InChI Key | SLBQXWXKPNIVSQ-UHFFFAOYSA-N |
| LogP | 0.9 |
| Synonyms |
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Applications:
UV-Vis Spectrum of 4-Nitrophthalic Acid
December 23, 2025
If you are looking for optimized HPLC method to analyze 4-Nitrophthalic Acid check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Separation of 3-Nitrophthalic and 4-Nitrophthalic Acids
December 6, 2007
| Column | Newcrom BH, 3.2×50 mm, 3 µm, 100A |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275nm |
| Class of Compounds | Acid |
| Analyzing Compounds | 3-Nitrophthalic and 4-Nitrophthalic Acids |
Nitrophthalic acid exist in the form of two isomers: 3-nitrophthalic and 4-nitrophthalic acid. Both forms have similar hydrophobicity and ionic properties. Isomers of nitrophthalic acid are separated on Primesep D column by combination of reverse phase and anion-exchange mechanisms. Method developed on a short-50 mm- column and allows fast analysis of these two isomers.
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options4-Nitrophthalic Acid
