HPLC Separation of 3-Nitrophthalic and 4-Nitrophthalic Acids

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Column Newcrom BH, 3.2x50 mm, 3 µm, 100A
Mobile Phase MeCN/H2O - 60/40%
Buffer H3PO4 - 0.5%
Flow Rate 0.5 ml/min
Detection UV 275nm


Class of Compounds Acid
Analyzing Compounds 3-Nitrophthalic and 4-Nitrophthalic Acids
Nitrophthalic acid exist in the form of two isomers: 3-nitrophthalic and 4-nitrophthalic acid. Both forms have similar hydrophobicity and ionic properties. Isomers of nitrophthalic acid are separated on Primesep D column by combination of reverse phase and anion-exchange mechanisms. Method developed on a short-50 mm- column and allows fast analysis of these two isomers.
Application Analytes:
3-Nitrophthalic Acid
4-Nitrophthalic Acid