Separation of Epinephrine and Norepinephrine

Primesep 200 separates the catecholamines, norepinephrine and epinephrine, less than 8 minutes. Norepinephrine and epinephrine are baseline resolved by a combination of reversed-phase, and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with UV detection at 210 nm.

Condition

Column Primesep 200, 3.2×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer  TFA – 0.05%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Norepinephrine, Epinephrine

 

Application Column

Primesep 200

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Epinephrine
Norepinephrine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.