HPLC Separation of Succinylcholine and Choline Using a Primesep 200 Column

 

Condition
Column Primesep 200, 4.6×50 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 0 – 20 %, 7 min
Buffer TFA – 0.05-0.20%
Flow Rate 1.0 ml/min
Detection ELSD 45C

 

Description
Class of Compounds
 Drug, Acid, Paralytic, Hydrophilic, Ionizable
Analyzing Compounds Succinic Acid, Succinylcholine, Choline

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Choline
Succinic Acid
Succinylcholine
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.