Primesep B separates tertiary amines, such as cetylpyridinium with symmetrical peak shape by a combination of reversed-phase and ion-exclusion mechanisms. The embedded basic functional group on the stationary phase shields the underlying silanols to prevent peak tailing. Retention time can be changed by changing either organic content or acid content in the mobile phase. C18 reversed-phase columns do not typically show this tuning ability with acid content. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 250 nm.
| Column | Primesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O |
| Buffer | TFA |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210nm |
| Class of Compounds |
Surfactant, Hydrophobic, Ionizable |
| Analyzing Compounds | Cetylpyridinium Chloride |
Application Column
Primesep B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Cetylpyridinium ChlorideApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
