Trisaccharides can be difficult to separate using conventional HPLC columns. The four trisaccharides panose, raffinose, isomaltotriose, and kestose have the same chemical formula, and the all have two glycosidic bonds connecting the three monosaccharides. Obelisc N was used as a stationary phase to separate trisaccharides because it is capable of multiple modes of separation. Obelisc N is a highly polar column that retains polar and charged analytes. Even though the trisaccharides differ only in regio- and stereochemistry they are resolved.
| Column | Obelisc R, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 75% |
| Buffer | AmFm pH 3.0- 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Trisaccharides, Hydrophilic, Ionizable |
| Analyzing Compounds | Raffinose, Kestose, Panose, Isomaltose |
Application Column
Obelisc N
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
IsomaltotrioseKestose
Panose
Raffinose
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
