Panose

panose

CAS Number33401-87-5
Molecular FormulaC18H32O16
Molecular Weight504.438
InChI KeyZCLAHGAZPPEVDX-MQHGYYCBSA-N
LogP-7.2
Synonyms
  • alpha-D-glucopyranose, O-alpha-D-glucopyranosyl-(1-6)-O-alpha-D-glucopyranosyl-(1-4)
  • beta-D-glucopyranose, O-beta-D-glucopyranosyl-(1-6)-O-beta-D-glucopyranosyl-(1-4)
  • D-glucopyranose, O-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-6)
  • D-glucopyranose, O-beta-D-glucopyranosyl-(1-6)-O-beta-D-glucopyranosyl-(1-4)
  • glucopyranoside, O-alpha-D-glucopyranosyl-(1-6)-alpha-D-glucopyranosyl, beta-D
  • glucopyranoside, O-alpha-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl, alpha-D
  • glucopyranoside, O-alpha-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl, beta-D
  • glucosyl-alpha-(1,6)-glucosyl-alpha-(1,4)-glucose
  • panose

Applications:

Separation of Trisaccharides on Obelisc N Column

July 6, 2015

 

Trisaccharides can be difficult to separate using conventional HPLC columns. The four trisaccharides panose, raffinose, isomaltotriose, and kestose have the same chemical formula, and the all have two glycosidic bonds connecting the three monosaccharides. Obelisc N was used as a stationary phase to separate trisaccharides because it is capable of multiple modes of separation. Obelisc N is a highly polar column that retains polar and charged analytes. Even though the trisaccharides differ only in regio- and stereochemistry they are resolved.

Condition 
Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 75%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection ELSD

Description
Class of Compounds
 Trisaccharides,  Hydrophilic, Ionizable
Analyzing Compounds Raffinose, Kestose, Panose, Isomaltose

 

Application Column

Obelisc N

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Isomaltotriose
Kestose
Panose
Raffinose
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.