| CAS Number | 521-18-6 |
|---|---|
| Molecular Formula | C19H30O2 |
| Molecular Weight | 290.447 |
| InChI Key | NVKAWKQGWWIWPM-ABEVXSGRSA-N |
| LogP | 3.55 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Dihydrotestosterone on Primesep 100 Column
October 4, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of 5alpha-Dihydrotestosterone on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of 5alpha-Dihydrotestosterone
Dihydrotestosterone (DHT) is an androgen hormone derived from testosterone through the action of the enzyme 5-alpha-reductase. It plays a crucial role in the development of male characteristics, including facial and body hair growth, deepening of the voice, and muscle development. Due to its potent androgenic effects, elevated levels of DHT are associated with conditions such as androgenetic alopecia (male pattern baldness) and benign prostatic hyperplasia (enlarged prostate).
5alpha-Dihydrotestosterone can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric acid as a buffer. This method allows for detection using UV 200 nm.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 50% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 |
| Sample | 0.1 mg/mL |
| Diluent | MeCN/H2O- 50/50% |
| LOD* | 125 ppb |
| Class of Compounds | Hormone |
| Analyzing Compounds | 5alpha-Dihydrotestosterone |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Separation of 5alpha-Dihydrotestosterone on Newcrom R1 HPLC column
May 16, 2018
5alpha-Dihydrotestosterone can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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