HPLC Method for Analysis of Dihydrotestosterone on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of 5alpha-Dihydrotestosterone on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of 5alpha-Dihydrotestosterone on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of 5alpha-Dihydrotestosterone

Dihydrotestosterone (DHT) is an androgen hormone derived from testosterone through the action of the enzyme 5-alpha-reductase. It plays a crucial role in the development of male characteristics, including facial and body hair growth, deepening of the voice, and muscle development. Due to its potent androgenic effects, elevated levels of DHT are associated with conditions such as androgenetic alopecia (male pattern baldness) and benign prostatic hyperplasia (enlarged prostate).

5alpha-Dihydrotestosterone can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric acid as a buffer. This method allows for detection using UV 200 nm.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 50%
BufferH3PO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 200
Sample0.1 mg/mL
DiluentMeCN/H2O- 50/50%
LOD*125 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Hormone
Analyzing Compounds5alpha-Dihydrotestosterone

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
5alpha-Dihydrotestosterone
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.