| CAS Number | 46817-91-8 |
|---|---|
| Molecular Formula | C13H19NO3 |
| Molecular Weight | 237.299 |
| InChI Key | YWPHCCPCQOJSGZ-UHFFFAOYSA-N |
| LogP | 1.34 |
| Synonyms |
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Applications:
HPLC Chiral Separation of Viloxazine Enantiomers on Cyralis CL Column
September 15, 2026
HPLC Chiral Method for Analysis of Viloxazine Enantiomers on Cyralis CL Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Viloxazine.
Viloxazine is a selective norepinephrine reuptake inhibitor with the chemical formula C13H19NO3. It is used in treatment of attention deficit hyperactivity disorder (ADHD). Originally, it was used to treat depression, but was discontinued. You can find detailed UV spectra of Viloxazine and information about its various lambda maxima by visiting the following link.
This application describes a normal-phase chiral HPLC method for the separation of Viloxazine enantiomers using SIELC’s Cyralis CL chiral column. Cyralis CL is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.
Viloxazine can be retained and analyzed using the Cyralis CL stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of sodium perchlorate and acetonitrile. Detection is performed using UV.
Condition
| Column | Cyralis CL, 4.6 x 100 mm, 5 µm, 100 A, normal phase |
| Mobile Phase | Hexane/Ethanol/Ethanolamine – 50/50/0.5% |
| Flow Rate | 0.5 mL/min |
| Detection | UV 230 nm |
| Resolution | 2.14 |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Viloxazine |
Application Column
Cyralis CL
Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase
Uv-Vis Spectrum of Viloxazine
February 17, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Viloxazine check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
Separation of Viloxazine on Newcrom R1 HPLC column
February 16, 2018
Viloxazine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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