| CAS Number | 58-22-0 |
|---|---|
| Molecular Formula | C19H28O2 |
| Molecular Weight | 288.431 |
| InChI Key | MUMGGOZAMZWBJJ-DYKIIFRCSA-N |
| LogP | 3.32 |
| Synonyms |
|
Applications:
HPLC MS Method for Analysis of Testosterone on Primesep B Column
October 4, 2024
High Performance Liquid Chromatography (HPLC) MS Method for Analysis of Testosterone on Primesep B by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Testosterone
Testosterone is the primary male sex hormone, though it is also present in smaller amounts in females. It plays a key role in the development of male reproductive tissues such as the testes and prostate, as well as promoting secondary sexual characteristics like increased muscle and bone mass, and the growth of body hair. In addition to its role in reproduction and physical development, testosterone is also important for general health and well-being.
Testosterone can be retained, separated and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV 270 nm.
You can find detailed UV spectra of Testosterone and information about its various lambda maxima by visiting the following link.
| Column | Primesep B, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 35% |
| Buffer | Ammonium Formate pH 3.0 – 20 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV 270 nm, ESI SIM 289+ |
| Sample | 0.01 mg/ml |
| Diluent | MeCN/H2O- 50/50% |
| LOD* | 5 ppb |
| Class of Compounds | Hormone |
| Analyzing Compounds | Testosterone |
Application Column
Primesep B
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
LC MS Detection
Separation of Testosterone on Newcrom R1 HPLC column
May 16, 2018
Testosterone is an anabolic steroid which is the primary male sex hormone. It is produced naturally by both males and females; however, males produce much more than females. Testosterone supplements can be used if there is not enough testosterone in the body. Testosterone can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options