Stevioside structural formula

CAS Number57817-89-7
Molecular FormulaC38H60O18
Molecular Weight804.881
  • Stevioside
  • 1-O-{(5beta,8alpha,9beta,10alpha,13alpha)-13-[(2-O-beta-D-Glucopyranosyl-beta-D-glucopyranosyl)oxy]-18-oxokaur-16-en-18-yl}-beta-D-glucopyranose
  • 57817-89-7
  • 4-17-00-03618
  • Kaur-16-en-18-oic acid, 13-[(2-O-?-D-glucopyranosyl-?-D-glucopyranosyl)oxy]-, ?-D-glucopyranosyl ester, (4?)-
  • (4?)-?-D-glucopyranosyl 13-[(2-O-?-D-glucopyranosyl-?-D-glucopyranosyl)oxy]kaur-16-en-18-oate
  • (4?)-?-D-Glucopyranosyl-13-[(2-O-?-D-glucopyranosyl-?-D-glucopyranosyl)oxy]kaur-16-en-18-oat
  • 13-[(2-O-?-D-glucopiranosil-?-D-glucopiranosil)oxi]kaur-16-en-18-oato de (4?)-?-D-glucopiranosilo
  • 13-[(2-O-?-D-glucopyrannosyl-?-D-glucopyrannosyl)oxy]kaur-16-ene-18-oate de (4?)-?-D-glucopyrannosyle
  • Steviosin
  • ?-G Sweet PX
  • ?-G-Sweet
  • BRN 0077427
  • EINECS 260-975-5


Separation of Stevioside on Newcrom R1 HPLC column

February 19, 2018
Separation of Stevioside on Newcrom C18 HPLC column

Stevioside can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: or by phone: 847-229-2629.