| CAS Number | 124-07-2 |
|---|---|
| Molecular Formula | C8H16O2 |
| Molecular Weight | 144.215 |
| InChI Key | WWZKQHOCKIZLMA-UHFFFAOYSA-N |
| LogP | 3.05 |
| Synonyms |
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Applications:
HPLC Determination of Octanoic acid (Caprylic acid) on Primesep 100 Column
June 1, 2021
HPLC Method for Octanoic acid (Caprylic acid) on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Octanoic acid (Caprylic acid).
Octanoic acid (Caprylic acid) is the common name for the eight-carbon straight-chain fatty acid. It can be found in numerous foods such as coconuts, cherries, plums, pineapple sages, black raspberries, and shallots. Octanoic acid is found to be associated with medium-chain acyl-CoA dehydrogenase deficiency, a genetic disorder that affects the metabolism of converting certain fatty acids to energy.
Octanoic acid can be retained on a mixed-mode Primesep 100 column by using an isocratic method consisting of a 50/50% Acetonitrile (ACN) and water mobile phase with a Phosphoric acid (H3PO4) buffer. UV detection at 210 nm.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds |
Hydrophobic, Acid |
| Analyzing Compounds | Octanoic acid (Caprylic acid) |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Separation of Octanoic acid on Newcrom R1 HPLC column
February 16, 2018
Octanoic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options