HPLC Determination of Octanoic acid (Caprylic acid) on Primesep 100 Column

HPLC Method for Octanoic acid (Caprylic acid) on Primesep 100 by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Octanoic acid (Caprylic acid).

Octanoic acid (Caprylic acid) is the common name for the eight-carbon straight-chain fatty acid. It can be found in numerous foods such as coconuts, cherries, plums, pineapple sages, black raspberries, and shallots. Octanoic acid is found to be associated with medium-chain acyl-CoA dehydrogenase deficiency, a genetic disorder that affects the metabolism of converting certain fatty acids to energy.

Octanoic acid can be retained on a mixed-mode Primesep 100 column by using an isocratic method consisting of a 50/50% Acetonitrile (ACN) and water mobile phase with a Phosphoric acid (H3PO4) buffer. UV detection at 210 nm.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 50/50%
Buffer H3PO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 210 nm

 

Description
Class of Compounds
 Hydrophobic, Acid
Analyzing Compounds Octanoic acid (Caprylic acid)

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Octanoic acid (Caprylic acid)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.