HPLC Determination of Octanoic acid (Caprylic acid) on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode

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Octanoic acid (Caprylic acid) is the common name for the eight-carbon straight-chain fatty acid. It can be found in numerous foods such as coconuts, cherries, plums, pineapple sages, black raspberries, and shallots. Octanoic acid is found to be associated with medium-chain acyl-CoA dehydrogenase deficiency, a genetic disorder that affects the metabolism of converting certain fatty acids to energy. Octanoic acid can be retained on a mixed-mode Primesep 100 column by using an isocratic method consisting of a 50/50% Acetonitrile (ACN) and water mobile phase with a Phosphoric acid (H3PO4) buffer. UV detection at 210 nm.


Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer H3PO4 - 0.2%
Flow Rate 1.0 ml/min
Detection UV 210 nm


Class of Compounds Hydrophobic, Acid
Analyzing Compounds Octanoic acid (Caprylic acid)
Application Analytes:
Octanoic acid