Midodrine

Midodrine structural formula

CAS Number42794-76-3
Molecular FormulaC12H18N2O4
Molecular Weight254.286
InChI KeyPTKSEFOSCHHMPD-UHFFFAOYSA-N
LogP-0.189
Synonyms
  • Midodrine
  • N-[2-(2,5-Dimethoxyphenyl)-2-hydroxyethyl]glycinamide
  • Acetamide, 2-amino-N-[2-(2,5-dimethoxyphenyl)-2-hydroxyethyl]-
  • 42794-76-3
  • EINECS 255-945-3
  • 2-Amino-N-(2,5-dimethoxy-beta-hydroxyphenethylacetamide
  • (RS)-N'-(beta-Hydroxy-2,5-dimethoxy-phenethyl)glycinamid
  • Midodrin
  • 2-Amino-N-(2,5-dimethoxy-beta-hydroxyphenethyl)acetamid
  • DL-N1-(beta-Hydroxy-2,5-dimethoxyphenethyl)glycinamid
  • Midodrina
  • Midodrinum
  • UNII-6YE7PBM15H
  • (+-)-2-amino-N-(beta-hydroxy-2,5-dimethoxyphenethyl)acetamide
  • 1-(2',5'-Dimethoxyphenyl)-2-glycinamidoethanol
  • 2-Amino-N-(2,5-dimethoxy-beta-hydroxyphenethyl)acetamide
  • DL-N1-(beta-Hydroxy-2,5-dimethoxyphenethyl)glycinamid

Applications:

HPLC Chiral Separation of Midodrine Enantiomers on Cyralis CL Column

September 18, 2026

HPLC Chiral Method for Analysis of Midodrine Enantiomers on Cyralis CL Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Midodrine.

Midodrine is an a prescription oral medication with the chemical formula C12H18N2O4. It is used primarily in treatment of symptomatic orthostatic hypotension. It works through tightening blood vessels to raise blood pressure.

This application describes a normal-phase chiral HPLC method for the separation of Midodrine enantiomers using SIELC’s Cyralis CL chiral column. Cyralis CL is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Midodrine can be retained and analyzed using the Cyralis CL stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of sodium perchlorate and acetonitrile. Detection is performed using UV.

Condition

ColumnCyralis CL, 4.6 x 100 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/1-Butylamine– 50/50/0.5%
Flow Rate1.0 mL/min
DetectionUV 290 nm
Resolution3.37

Description

Class of CompoundsDrug
Analyzing CompoundsMidodrine

Application Column

Cyralis CL

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Midodrine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Midodrine on Newcrom R1 HPLC column

February 16, 2018
Separation of Midodrine on Newcrom C18 HPLC column

Midodrine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Midodrine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.