HPLC Chiral Separation of Midodrine Enantiomers on Cyralis CL Column

HPLC Chiral Method for Analysis of Midodrine Enantiomers on Cyralis CL Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Midodrine.

Midodrine is an a prescription oral medication with the chemical formula C12H18N2O4. It is used primarily in treatment of symptomatic orthostatic hypotension. It works through tightening blood vessels to raise blood pressure.

This application describes a normal-phase chiral HPLC method for the separation of Midodrine enantiomers using SIELC’s Cyralis CL chiral column. Cyralis CL is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Midodrine can be retained and analyzed using the Cyralis CL stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of sodium perchlorate and acetonitrile. Detection is performed using UV.

Condition

ColumnCyralis CL, 4.6 x 100 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/1-Butylamine– 50/50/0.5%
Flow Rate1.0 mL/min
DetectionUV 290 nm
Resolution3.37

Description

Class of CompoundsDrug
Analyzing CompoundsMidodrine

Application Column

Cyralis CL

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Midodrine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.