Methiocarb

Methiocarb structural formula

CAS Number2032-65-7
Molecular FormulaC11H15NO2S
Molecular Weight225.310
InChI KeyYFBPRJGDJKVWAH-UHFFFAOYSA-N
LogP2.92
Synonyms
  • Methiocarb
  • 3,5-Dimethyl-4-(methylsulfanyl)phenyl methylcarbamate
  • Phenol, 3,5-dimethyl-4-(methylthio)-, methylcarbamate
  • 2032-65-7
  • Mercaptodimethur
  • Phenol, 3,5-dimethyl-4-(methylthio)-, methylcarbamate
  • 3,5-Dimethyl-4-(methylthio)phenyl methylcarbamate
  • 4-(Methylthio)-3,5-xylyl methylcarbamate
  • 4-(Methylthio)-3,5-xylyl-N-methylcarbamate
  • 4-Methylthio-3,5-dimethylphenyl methylcarbamate
  • Carbamic acid, methyl-, 4-(methylthio)-3,5-xylyl ester
  • mercaptodimetur
  • Mesurol
  • Mesurol phenol
  • Metmercapturon
  • Phenol, 3,5-dimethyl-4-(methylthio)-, 1-(N-methylcarbamate)
  • Bayer 37344
  • BRN 1881431
  • Carbamic acid, methyl-, 3,5-dimethyl-4-(methylthio)phenyl ester
  • Caswell No. 578B
  • 3,5-Dimethyl-4-methylmercaptophenyl-N-methyl-carbamate
  • 3,5-Dimethyl-4-(methylthio)phenol methylcarbamate
  • EINECS 217-991-2
  • EPA Pesticide Chemical Code 100501
  • Grandslam
  • Lizetan
  • Methiocarbe
  • Methyl carbamic acid 4-(methylthio)-3,5-xylyl ester
  • 4-Methylmercapto-3,5-dimethylphenyl N-methylcarbamate
  • 3,5-Xylenol, 4-(methylthio)-, methylcarbamate
  • 3,5-Dimethyl-4-methyl-thiophenyl-N-carbamat
  • UNII-JI9431OS31
  • 3,5-Dimethyl-4-methylthiophenyl N-methylcarbamate
  • 4-Methylmercapto-3,5-dimethylphenyl N-methylcarbamate
  • 4-Methylmercapto-3,5-xylyl methylcarbamate
  • 4-Methylthio-3,5-xylyl methylcarbamate
  • 716-16-5

Applications:

HPLC Method for Analysis of Methiocarb on Newcrom B Column on Alltesta™

January 8, 2026

HPLC Method for Methiocarb on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Methiocarb 

Methiocarb is a carbamate pesticide with the chemical formula C11H15NO2S. It works as a acetylcholinesterase inhibitor. It has been in use as an insecticide, bird repellent, acaricide, and molluscicide since 1960s.

Methiocarb can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.

ColumnNewcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferAmmonium Formate
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsPesticide
Analyzing CompoundsMethiocarb

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Methiocarb
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

UV-Vis Spectrum of Methiocarb

December 23, 2025
Access the UV-Vis Spectrum SIELC Library
UV-Vis Spectrum of Methiocarb.

If you are looking for optimized HPLC method to analyze Methiocarb check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Methiocarb
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Methiocarb on Newcrom R1 HPLC column

February 16, 2018
Separation of Methiocarb on Newcrom C18 HPLC column

Methiocarb can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Methiocarb
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.