| CAS Number | 96-82-2 |
|---|---|
| Molecular Formula | C12H22O12 |
| Molecular Weight | 358.296 |
| InChI Key | JYTUSYBCFIZPBE-AMTLMPIISA-N |
| LogP | -3.70 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Lactobionic Acid on Newcrom BH Column
May 7, 2024
HPLC Method for Analysis of Lactobionic acid on Newcrom BH Column by SIELC Technologies
Separation type: Liquid Chromatography Reversed-phase
Lactobionic Acid is a compound derived from lactose, a sugar found in milk. It is a bionic acid, which is a type of sugar acid that contains both a sugar and a carboxylic acid group. Lactobionic acid has applications in various industries, including pharmaceuticals, cosmetics, and food production, due to its properties such as antioxidant activity and its ability to chelate metal ions. In pharmaceuticals, it is used as an excipient in formulations, while in cosmetics, it is found in skincare products for its hydrating and exfoliating properties. Additionally, it is used as a food additive, particularly in processed foods, as a stabilizer and antioxidant.
Lactobionic Acid can be retained and analyzed on a reversed-phase Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and perchloric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 190 nm using UV detection
High Performance Liquid Chromatography (HPLC) Method for Analyses of Lactobionic acid on Newcrom BH Column by SIELC Technologies
Condition
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 80/20% |
| Buffer | HClO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 190 nm |
| Injection volume: | 3 µL |
| Sample | 1 mg/ml |
| Sample Diluent | MeCN/H2O – 50/50% |
| LOD* | 1 ppm |
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Lactobionic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Separation of Lactobionic acid on Newcrom R1 HPLC column
February 16, 2018
Lactobionic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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