HPLC Method for Analysis of Lactobionic Acid on Newcrom BH Column

HPLC Method for Analysis of Lactobionic acid on Newcrom BH Column by SIELC Technologies

Separation type: Liquid Chromatography Reversed-phase

HPLC Method for Analysis of Lactobionic acid on Newcrom BH Column by SIELC Technologies

Lactobionic Acid is a compound derived from lactose, a sugar found in milk. It is a bionic acid, which is a type of sugar acid that contains both a sugar and a carboxylic acid group. Lactobionic acid has applications in various industries, including pharmaceuticals, cosmetics, and food production, due to its properties such as antioxidant activity and its ability to chelate metal ions. In pharmaceuticals, it is used as an excipient in formulations, while in cosmetics, it is found in skincare products for its hydrating and exfoliating properties. Additionally, it is used as a food additive, particularly in processed foods, as a stabilizer and antioxidant.

Lactobionic Acid can be retained and analyzed on a reversed-phase Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and perchloric acid. This analytical method can be detected with high resolution and peak symmetry at a wavelength of 190 nm using UV detection

High Performance Liquid Chromatography (HPLC) Method for Analyses of Lactobionic acid on Newcrom BH Column by SIELC Technologies


ColumnNewcrom BH, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 80/20%
BufferHClO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 190 nm
Injection volume:3 µL
Sample1 mg/ml
Sample DiluentMeCN/H2O – 50/50%
LOD*1 ppm

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.


Class of CompoundsAcid
Analyzing CompoundsLactobionic acid

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Lactobionic acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.