| CAS Number | 492-27-3 |
|---|---|
| Molecular Formula | C10H7NO3 |
| Molecular Weight | 189.17 |
| InChI Key | HCZHHEIFKROPDY-UHFFFAOYSA-N |
| LogP | 0.3 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Kynurenic Acid on Primesep 100 Column
August 9, 2024
High Performance Liquid Chromatography (HPLC) Method for Analysis of Kynurenic Acid on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Kynurenic Acid
Kynurenic acid (KYNA) is a metabolite in the kynurenine pathway, which degrades the amino acid tryptophan. It acts as an antagonist to receptors in the brain, such as NMDA and alpha-7 nicotinic acetylcholine receptors, playing a dual role in the central nervous system. KYNA is neuroprotective by inhibiting excitatory neurotransmission and reducing oxidative stress, but elevated levels are linked to cognitive impairments and disorders like schizophrenia and depression.
Kynurenic Acid can be retained, separated and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs a gradient method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 240 nm.
You can find detailed UV spectra of Kynurenic Acid and information about its various lambda maxima by visiting the following link.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN from 5% to 50% |
| Buffer | Gradient H2SO4 from 0.1% to 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 240 nm |
| Class of Compounds | quinolinemonocarboxylic acid |
| Analyzing Compounds | Kynurenic Acid |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
UV-Vis Spectrum of Kynurenic Acid
July 19, 2024
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Kynurenic Acid check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
